Scientist - HTP Expression Purification
We are seeking a skilled molecular biologist and protein chemist at a Scientist level to join the high-throughput expression and purification group. The candidate will be expected to work on a variety of projects to purify antibodies from multiple species and heterologous expression systems using automated platforms.
General molecular biology involving isolation, manipulation, and characterization of DNA, RNA and proteins.
Design of mammalian cell expression vectors for recombinant proteins.
Mammalian cell culture and transfection using viral and lipid transfection systems.
Purify antibodies using variety of systems (protein A/G/L and ion exchange).
Analyze antibodies using automated protein characterization methods (SDS-PAGE/CE-SDS, SEC-MALLS, endotoxin, etc.).
Clone, express, and purify antibodies using automated systems at small to medium-scale.
Perform experiments with strong attention to detail and documentation.
Excellent written and oral skills are necessary.
Accomplish goals under project team time lines.
PhD in Biochemistry, Structural Biology, or related field with 0-5 years of industrial experience.
Demonstrated experience in design and construction of cell expression plasmid constructs, site-directed mutagenesis, and recombinant protein vector design.
Experience in expression of antibodies from small to medium scale is required.
Experience using automation and programming jobs on automation workstations is highly desirable.
Excellent time management, record keeping and organizational skills.
Demonstrated ability to work in a multidisciplinary team.
Associated topics: aseptic technique, biomedical, cytometry, dietician, drug, immuno oncology, nephrology, nutrition, vaccine, virus